Protein Method Blocking Buffers
Abcam Capsazepine, TRPV1 antagonist, 10MG
MW 376.9 Da, Purity >98%. TRPV1 vanilloid receptor antagonist (IC₅₀ = 562 nM). Analgesic in vivo.
The product is subject to the following: Abcam Restricted Use Statement
Amsbio LLC ChonBlock™ Blocking/Sample Dilution ELISA Buffer- 100 ml
ChonBlock Blocking/Sample Dilution ELISA Buffer, 100 ml
Cell Signaling Technology Phosphate Buffered Saline with Tween 20 (PBST-20X) 1000 ml
Phosphate Buffered Saline with Tween 20 (PBST-20X) 1000 ml
LAB BIOREAGENTS StrongBlock III Buffer 250ml
Lab Bioreagents StrongBlocks (I, II, III) are newly developed blocking buffers that provides exceptional performance and broad compatibility for Western blot, ELISA, IHC and nucleic acid detection assays The blocking buffer is to block excess binding sites on the micro-plate for reducing non-specific binding signal, by which the assay sensitivity and specificity are improved. As many factors can influence nonspecific binding, there is no single blocking buffer that is ideal for each assay. For example, depending on the recognition of detection probes from different assays, one individual blocking buffer may cross-react with detection probe. StrongBlocks (I, II, III) are a variety of buffers designed by different blocker components. During the assay, the test different blocking buffers helps to develop a immunoassay with low background noise and high signal-to-noise.
Bioworld Blotto/Antifoam in TBS, 500 mL
Blotto/Antifoam in TBS Blot blocking buffer used to block non-specific binding site for antibodies on the membrane Technical Information: 5% nonfat dry milk, 0.01% Anti-Foam A, 0.02% Sodium azide in TBS
Bioworld Blotto/Tween in PBS, 250 mL
Blotto/Antifoam in PBS Blot blocking buffer used to block non-specific binding site for antibodies on the membrane Technical Information: 5% nonfat dry milk, 0.05% Tween, 0.02% Sodium azide in PBS
Biotang Inc BLOTTO Antifoam In PBS (with azide), 250 ml
BLOTTO Antifoam In PBS (with azide), 250 ml.
Biotang Inc BLOTTO TWEEN IN PBS 250 ML, IBB-151, 5% nonfat dry milk with 0.05% Tween-20 in PBS (10 mM Sodium phosphate, 2 mM potassium phosphate,10 mM KCl,137 mM NaCl), 7.4 +/- 0.2. Prepared in 18.2 megohms water and filtered through 1 micron filter.
BLOTTO TWEEN IN PBS 250 ML, IBB-151, 5% nonfat dry milk with 0.05% Tween-20 in PBS (10 mM Sodium phosphate, 2 mM potassium phosphate,10 mM KCl,137 mM NaCl), 7.4 +/- 0.2. Prepared in 18.2 megohms water and filtered through 1 micron filter.
Biotang Inc BLOTTO/Tween 20 in TBS (with azide), 250 ml
BLOTTO/Tween 20 in TBS (with azide), 250 ml.
Biotang Inc BLOTTO/TWEEN 20 IN TBS 250 ML, IBB-161. 5% nonfat dry milk, 0.05% Tween-20 in TBS (50 mM Tris-HCl and 150 mM NaCl, pH 7.4 +/- 0.2). Prepared in 18.2 megohms water and filtered through 1 micron filter.
BLOTTO/TWEEN 20 IN TBS 250 ML, IBB-161. 5% nonfat dry milk, 0.05% Tween-20 in TBS (50 mM Tris-HCl and 150 mM NaCl, pH 7.4 +/- 0.2). Prepared in 18.2 megohms water and filtered through 1 micron filter.
Kd Medical Inc PBS 20X + TWEEN 20, 1 L
Concentrated 20X PBS and Tween 20 used to be diluted to a 1X working solution. Used as a wash buffer in various laboratory applications. One 1 Liter bottle.
Rockland Immunochemicals Blocking Buffer for Fluorescent Western Blotting - MB-070S
Western Blot Blocking Buffer is specifically designed for western blotting using fluorochrome conjugated antibodies. Pure nitrocellulose membrane is recommended for maximum performance. Other membranes, such as PVDF or nitrocellulose embedded in a support can be used, but may generate elevated backgrounds. Protein should be transferred from gel to membrane using standard protocols. This buffer can be used for membrane blocking and to dilute both primary and secondary antibodies. See www.rockland-inc.com for specific protocols. This product is suitable for use with fluorescent western blot imaging systems produced by Bio-Rad Laboratories, GE Healthcare, Alpha Innotech, FujiFilm Life Science, Licor Biosciences, UVP and Syngene.
Rockland Immunochemicals BLOTTO B PRE-MIXED - for a 1X SOLUTION of Immunoanalytical Grade 1% NON-FAT DRY MILK, 1% BSA in TBS with TWEEN-20 - B553-0500
Rockland Immunochemicals produces the following blocking buffer reagents: BSA, BLOTTO, BLOTTO A, BLOTTO B and Blocking Buffer for Fluorescent Western Blotting (see related products below). These products are intended to block non-specific binding of proteins in various immunological assays. Typically, western blotting and ELISA methods call for the addition of a blocking agent prior to and during incubation with primary antibody.
Zymo Research Corporation 5-mC ELISA Buffer, 250 ml
5-mC ELISA Buffer 250 ml. For use in the 5-mC DNA ELISA kit for DNA methylation quantification. Sold individually or as part of a complete ELISA kit.