Protein Method Blocking Buffers
Seracare Life Sciences Inc 10X TBS W/ 0 5% TWEEN 20(TBST)
10X Tris-buffered Saline (TBS) with 0.5% Tween 20 is used as a common Western blot wash solution and general wash buffer in immunoassay applications. Tween 20, a non-ionic detergent, reduces non-specific binding and protein-protein interaction during the wash step in immunoassay procedures.
Seracare Life Sciences Inc 10X PBS W/ TWEEN 20 (PBST) 1L
10X Phosphate-buffered Saline with Tween 20 (PBST) is ideal for use as a wash buffer in general immunoassay procedures. Tween 20, a non-ionic detergent, reduces non-specific binding and protein-protein interaction during the wash step in immunoassay procedures. pH=7.4. Conductivity controlled.
Biotium 10X Fish Gelatin Blocking Agent
10X Fish Gelatin Blocking Agent is a solution of cold water fish skin gelatin that can be added to blocking buffers to minimize non-specific antibody binding in immunodetection procedures such as Western Blotting and immunostaining. Unlike BSA or milk, fish gelatin does not contain IgG or serum proteins that could cross-react with mammalian antibodies. Fish gelatin can be used to block non-specific binding sites on positively charged nylon or PVDF membranes or used for blocking and dilution of antibodies during immunostaining procedures.
Biotium TrueBlack IF Background Suppressor System (Permeabilizing)
The TrueBlack Background Suppressor System (Permeabilizing) is a buffer system designed for blocking background from both non-specific antibody binding as well as direct interaction of fluorescent dyes on antibodies with cells or tissue sections.
Biocare Medical, LLC Peroxidazed 1 - 25 ml
Peroxidazed 1 is a highly stable form of hydrogen peroxide for blocking endogenous peroxidase. It is very effective for blocking non-specific staining in red blood cells. It is non-flammable, safer and less toxic when compared to hydrogen peroxide/methanol formulations. It can also be used on an automated staining system.
Biocare Medical, LLC Mouse-on-Mouse HRP-Polymer Bundle - 6.0 ml
The Mouse-on-Mouse HRP-Polymer is specially designed for using mouse primary antibodies on mouse tissues with minimum cross-reactivity to endogenous mouse IgG. Utilization of this goat anti-mouse horseradish peroxidase (HRP) polymer is advantageous over traditional biotinylation methods. Biotinylated anti-mouse secondary antibodies can bind to endogenous mouse IgG in the tissue in addition to the primary antibody, leading to troublesome background staining. Biocare Medical's biotin-free polymer technology provides the elimination of endogenous biotin, reduction of IHC steps and increased sensitivity.
Alkali Scientific BSA Blocking Buffer Solution in PBS with Azide, 200 Milliliters
BSA Blocking Buffer Solution in PBS with Azide is a protein blocking buffer used in molecular biology and biochemistry applications. This 200mL solution is ideal for blocking non-specific binding sites during immunoassays such as Western blotting, ELISA, and immunohistochemistry. The buffer contains Bovine Serum Albumin (BSA) to prevent nonspecific binding of antibodies or other proteins to the membrane, ensuring clearer and more reliable results. PBS provides a stable pH and isotonic conditions for the samples, while azide acts as a preservative to extend the shelf life of the solution. This product is essential in protein analysis and detection experiments.
Alkali Scientific Dry Powder Milk, 100 G
Dry Powder Milk, 100 G, is a non-fat milk powder used primarily in microbiological culture media and as a nutrient source in laboratory applications. It is commonly employed in the preparation of media for the growth of microorganisms, particularly those requiring a dairy-based nutrient source. The powder is utilized in various applications, including culturing bacteria and fungi, and in fermentation processes. This 100g package size is ideal for small-scale culture media preparation, ensuring precise control over the concentration of nutrients used in experiments. The product is highly versatile and essential in the preparation of high-quality, reproducible media formulations in research laboratories.
Bioworld Blotto/Tween in TBS, 250 mL
Blotto/Tween in TBS Blot blocking buffer used to block non-specific binding site for antibodies on the membrane Technical Information: 5% nonfat dry milk, 0.05% Tween-20, 0.02% Sodium azide in TBS
Crescent Chemical Co Inc Denhardt's Solution, 50X Concentration, 10mL
Denhardt's Solution, 50X Concentration, 10mL, Molecular Biology Grade, DNase/RNase not detected.Suitable for nucleic acid hybridization. Denhardt's solution is a mixture of blocking agents capable of saturating non-specific binding sites and to be used in membrane-based hybridization protocols. It is recommended for use with nylon membranes. Storage Conditions: -15 °C TO -25 °C
